Induction of human peripheral blood lymphocyte activation by supernatant of sonicated Actinomyces viscosus.
نویسندگان
چکیده
منابع مشابه
The influence of mitogen costimulation on the human lymphocyte blastogenic response to Actinomyces viscosus ultrasonicates.
Human peripheral blood lymphocytes were cultured for 48 hr in the presence of an ultrasonicate supernatant fraction of A~~tinonr.vces r~i~c~os~,s (AV-SUP). After washing to remove AV-SUP, the lymphocytes were cultured in the presence of concanavalin A or pokeweed mitogen for an additional 72 hr. Measurement of [,‘H]thymidine incorporation into DNA indicated that AV-SUP preculture resulted in an...
متن کاملSurface fibrils (fimbriae) of Actinomyces viscosus T14V.
Surface antigens of Actinomyces viscosus T14V were released from cell walls by digestion with lysozyme. These were separated by ion-exchange and gel filtration chromatography into fractions rich in carbohydrate or protein. The former contained a polysaccharide high in 6-deoxytalose, along with a peptide fragment from the cell wall. In the protein-rich fractions, material of high molecular weigh...
متن کاملMannose-contaminating agglutinin for Actinomyces viscosus and Actinomyces naeslundii.
Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial...
متن کاملRegulation of invertase of Actinomyces viscosus.
The regulation of diethylaminoethyl-partially purified invertase (EC 3.2.1.26; beta-D-fructofuranoside fructohydrolase) from the 37,000 X g-soluble intracellular fluid of Actinomyces viscosus serotype 2 strain M-100 was studied. Glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. Fructose-6-pho...
متن کاملEstablishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity.
The intraoral establishment and proportional distribution of suspected periodontal pathogens Actinomyces viscosus and Actinomyces naeslundii were studied using a recently developed differential plating medium, CNAC-20. Saliva and dental plaque samples were collected from 108 subjects ranging in age from infants to young adults; tongue and buccal mucosa samples were collected from only the adult...
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ژورنال
عنوان ژورنال: Japanese Journal of Oral & Maxillofacial Surgery
سال: 1987
ISSN: 2186-1579,0021-5163
DOI: 10.5794/jjoms.33.32